Although the evaluation of European Union regional policy is necessary to improve the effectiveness of the operational programmes, it is not usual to find studies comparing the efficiency of two programming periods for the case of a particular region. This could be explained by the fact that, at regional level, the study of the efficiency of the European funds during two different programming periods faces many different problems; for example, it entails the consideration of both adequate and homogeneous data, and similar methodology. The goal of this paper is twofold. The first goal is to estimate the economic impact of the European Union structural and cohesion funds received by Extremadura, a Spanish convergence objective NUTS II region, during the programming period 2007-2013. To this end, it is provided a multipliers analysis based on a Social Accounting Matrix (SAM) of Extremadura for the year 2000. Secondly, the paper will compare the returns obtained in terms of output and employment by the European funds received in Extremadura during the periods 2000-2006 and 2007-2013. Our results allow quantifying the effects of the EU regional policy, showing and comparing the efficiency for these two programming periods.