This paper aims to reveal the role of entrepreneurial universities in the regional economic development of Türkiye. By using spatial econometrics tools including cluster maps, namely LISA and Geary, and spatial regression models for NUTS 3 regions (81 provinces), the effect of the entrepreneurial universities on regional development indicators, i.e., gross domestic product (GDP), GDP per capita (GDPPC), and the number of enterprises is examined from 2012 to 2017. Both the number of entrepreneurial universities and their presence is effective on all three regional development indicators. Additionally, investing in education, promoting export activities, and developing physical infrastructure should be prioritized in provincial development plans to overcome regional disparities.